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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
02/12/2021 |
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Data da última atualização: |
02/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, P. A.; PACHECO, T. F.; CAMARGO, B. R. de; MARCO, J. L. de; SALUM, T. F. C. |
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Afiliação: |
PEDRO ALVES MARTINS, Universidade de Brasília (UnB); THALYTA FRAGA PACHECO, CNPAE; BRENDA RABELLO DE CAMARGO; JANICE LISBOA DE MARCO, Universidade de Brasília (UnB); THAIS FABIANA CHAN SALUM, CNPAE. |
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Título: |
Solid-state fermentation production and characterization of an alkaline lipase from a newly isolated Burkholderia gladioli strain. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Preparative Biochemistry & Biotechnology, 3, p. 1-10, May 2021. |
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DOI: |
https://doi.org/10.1080/10826068.2021.1910959 |
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Idioma: |
Inglês |
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Conteúdo: |
The newly isolated Burkholderia gladioli BRM58833 strain was shown to secrete an alkaline lipase highly active and stable in organic solvents. Lipase production was optimized through the cultivation of the strain by solid-state fermentation in wheat bran. The lipase extraction conditions were also optimized. T. Proteomic analysis revealed the optimized extract is composed of two esterases and three true lipases, showing a preference for long-chain substrates. The highest activity was obtained at 50 °C and pH 9. However, the extract maintained more than 50% of its maximum activity between pH 8.0 and 10.0 and throughout the whole temperature range evaluated (32?70 °C). The enzymes were inhibited by SDS, EDTA, ZnSO4 and FeCl3 and activated by FeSO4, MgCl2 and BaCl2. The lipases conserved their activity when incubated in solvents as acetonitrile, diethyl ether, n-heptane n-hexane, toluene, methanol and t-butanol. The resistance of these lipases to solvents and expressive thermostability when compared to other lipases, reveal their potential both in hydrolysis reactions and in synthesis of esters. |
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Thesagro: |
Fermentação; Lípase. |
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Thesaurus Nal: |
Burkholderia gladioli; Process monitoring; Solid state fermentation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01928naa a2200241 a 4500
001 2137028
005 2021-12-02
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/10826068.2021.1910959$2DOI
100 1 $aMARTINS, P. A.
245 $aSolid-state fermentation production and characterization of an alkaline lipase from a newly isolated Burkholderia gladioli strain.$h[electronic resource]
260 $c2021
520 $aThe newly isolated Burkholderia gladioli BRM58833 strain was shown to secrete an alkaline lipase highly active and stable in organic solvents. Lipase production was optimized through the cultivation of the strain by solid-state fermentation in wheat bran. The lipase extraction conditions were also optimized. T. Proteomic analysis revealed the optimized extract is composed of two esterases and three true lipases, showing a preference for long-chain substrates. The highest activity was obtained at 50 °C and pH 9. However, the extract maintained more than 50% of its maximum activity between pH 8.0 and 10.0 and throughout the whole temperature range evaluated (32?70 °C). The enzymes were inhibited by SDS, EDTA, ZnSO4 and FeCl3 and activated by FeSO4, MgCl2 and BaCl2. The lipases conserved their activity when incubated in solvents as acetonitrile, diethyl ether, n-heptane n-hexane, toluene, methanol and t-butanol. The resistance of these lipases to solvents and expressive thermostability when compared to other lipases, reveal their potential both in hydrolysis reactions and in synthesis of esters.
650 $aBurkholderia gladioli
650 $aProcess monitoring
650 $aSolid state fermentation
650 $aFermentação
650 $aLípase
700 1 $aPACHECO, T. F.
700 1 $aCAMARGO, B. R. de
700 1 $aMARCO, J. L. de
700 1 $aSALUM, T. F. C.
773 $tPreparative Biochemistry & Biotechnology, 3, p. 1-10, May 2021.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
06/10/2006 |
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Data da última atualização: |
11/10/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
VIEIRA, R. F.; VIDAL PEREZ, D.; SILVA, C. M. M. S. |
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Afiliação: |
ROSANA FARIA VIEIRA, CNPMA; D. VIDAL PEREZ; C. M. M. de S. SILVA, Embrapa Meio Ambiente. |
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Título: |
Avaliação da concentração de metais pesados em grãos de soja e feijão cultivados em solo suplementado com lodo de esgoto. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: CONGRESO VIRTUAL IBEROAMERICANO SOBRE GESTIÓN DE CALIDAD EN LABORATORIOS, 2., 2003, Valladolid. Resúmenes... Valladolid: ITACYL, 2003. |
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Idioma: |
Português |
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Thesagro: |
Adubo de esgoto; Feijão; Lodo residual; Metal pesado; Soja; Solo. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/12274/1/artcongresA69.pdf
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Marc: |
LEADER 00699nam a2200193 a 4500
001 1015123
005 2017-10-11
008 2003 bl uuuu u00u1 u #d
100 1 $aVIEIRA, R. F.
245 $aAvaliação da concentração de metais pesados em grãos de soja e feijão cultivados em solo suplementado com lodo de esgoto.$h[electronic resource]
260 $aIn: CONGRESO VIRTUAL IBEROAMERICANO SOBRE GESTIÓN DE CALIDAD EN LABORATORIOS, 2., 2003, Valladolid. Resúmenes... Valladolid: ITACYL$c2003
650 $aAdubo de esgoto
650 $aFeijão
650 $aLodo residual
650 $aMetal pesado
650 $aSoja
650 $aSolo
700 1 $aVIDAL PEREZ, D.
700 1 $aSILVA, C. M. M. S.
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Embrapa Meio Ambiente (CNPMA) |
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